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Journal: Nature Communications
Article Title: Grb2 monomer–dimer equilibrium determines normal versus oncogenic function
doi: 10.1038/ncomms8354
Figure Lengend Snippet: Using the pY160 antibody IHC was performed on multi-tumours tissue microarray of 95 samples with 40 types of tumours from 27 organs. ( a ) Weak or no staining for Grb2 Y160 phosphorylation in grade I colon adenocarcinoma. ( b ) Strong staining for Grb2 Y160 phosphorylation in grade III colon adenocarcinoma. ( c ) Weak or no staining for Grb2 Y160 phosphorylation in grade II prostate adenocarcinoma. ( d ) Moderate staining for Grb2 Y160 phosphorylation in grade III prostate adenocarcinoma. A significant increase in the level of pY160 phosphorylation is seen in higher-grade tumour samples. The bars on a–d correspond to 50 μm. ( e ) The pY160 antibody staining patterns for 118 colon cancer tissue samples. The samples were scored according to the pY160 staining as weak or none, moderate and strong and plotted against tumour grade as percentage. Data compiled from normal tissues ( n =42), and tumours grade I ( n =28), grade II ( n =34) and grade III ( n =14) which shows a progressive increase in the strength of pY160 staining with higher tumour grade. ( f ) The pY160 antibody staining patterns for 42 clinical prostate cancer tissue samples with the relative staining patterns for pY160 antibody. As above, samples were scored, sorted and plotted against tumour grade as percentage. Normal and/or hyperplasic tissue ( n =15), tumour grade II ( n =13) and grade III ( n =14). Here the pY160 staining is only associated with malignant tumours and intensity of staining is increased in tumours with a higher level of malignancy.
Article Snippet:
Techniques: Microarray, Staining, Phospho-proteomics